响应面法优化鲟鱼精蛋白的提取工艺
Optimization of extraction process of sturgeon sperm protein by response surface methodology
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摘要: 以鲟鱼-甲骨板的精巢组织为原料,从中提取鱼精蛋白粗品,建立硫酸提取分离鱼精蛋白的最优工艺。以蛋白得率为指标,通过单因素实验研究硫酸浓度、液料比、提取温度、提取时间和提取次数的影响大小,初步确定鱼精蛋白的最佳分离条件。进一步利用响应面软件Box-Behnken设计模型确定鲟鱼精蛋白的最佳提取工艺条件,结果表明:硫酸浓度0.83 mol/L,液料比6.2∶1 m L/g,提取时间0.5 h,提取温度35℃,提取次数4次。经验证,最优工艺条件下鱼精蛋白的得率平均达到11.28%,且纯度高达86.13%,分子量约为10 ku,有良好的抑菌活性,金黄色葡萄球菌抑菌圈直径达15.75 mm。Abstract: The extraction and separation of protamine from sturgeon by H2SO4 was studied and the optimal extraction process was set up.Taking the protein yield as the main indicator,the optimal separating conditions were initial determined on the basis of single factor tests on the influence of the concentration and amount of H2SO4,extraction temperature,extraction duration and extraction times.The best extraction conditions were finally confirmed with the Box- Behnken design in Design- Expert software:the concentration of H2SO4 was 0.83 mol / L while the amount was 6.2∶ 1 times volume,the extraction temperature was better at35 ℃ and extracting 0.5 h for 4 times was recommended. It proved that the mean yield of protamine was 11.28% under the optimal extraction conditions.Moreover,the extracted protamine has been shown to have not only high purity of 86.13% and low molecular weight of 10 ku but also good antibacterial activity against staphylococcus aureus with the inhibition zone diameter of15.75 mm.
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