超声辅助原位生成离子液体分散液相微萃取结合高效液相色谱法分析硫酸沙丁胺醇和盐酸莱克多巴胺
Analysis of salbutamol sulfate and ractopamine hydrochloride with ultrasonic assisted in-situ ionic liquid dispersive liquid phase microextraction and HPLC
-
摘要: 目的:建立超声辅助原位生成离子液体分散液相微萃取（UAIS-IL-DLPME）结合HPLC分析硫酸沙丁胺醇（SAL）和盐酸莱克多巴胺（RAC）的方法。方法:采用绿色环保的亲水性离子液体1-己基-3-甲基咪唑四氟硼酸盐C6MIMBF4和离子交换试剂双三氟甲烷磺酰亚胺锂Li NTF2,超声辅助原位生成C6MIMNTF2,目标化合物SAL、RAC同时被萃取、富集到新生成的疏水性离子液体相中,从而达到萃取、分离和富集的效果。结果:确定最佳的萃取条件是:萃取溶剂为20μLC6MIMBF4、离子交换剂为Li NTF2、盐浓度为4%、分散剂为30μL四氢呋喃、超声振荡时间为35 min,离心速度和时间分别为12000 r/min和10 min,SAL和RAC的富集倍数分别为42.3和39.6,线性范围10100 ng/mL。结论:UAIS-IL-DLPME结合高效液相色谱法进行分析,达到了在饲料及动物内脏、肌肉样品中对目标化合物SAL、RAC富集、净化和快速检测。Abstract: Objective: To bring forth a method that analyzes salbutamol sulfate and ractopamine hydrochloride with Ultrasonic assisted in-situ ionic liquid dispersive liquid phase microextraction( UAIS-IL-DLPME) and HPLC.Method: the environmentally-friendly ionic liquidC_6MIMBF_4,ion exchange agent Li NTF_2 and in-situ formationC_6MIMNTF_2were exploited to extract and concentrate the targeted compounds RAC and SAL in the newly generated hydrophobic ionic liquid for the sake of extraction,separation and concentration. Result: The optimal extraction conditions were determined as follows: extraction solvent for 20 μLC_6MIMBF_4,ion exchanger for Li NTF_2,salt concentration for 4%,dispersant for 30 μL tetrahydrofuran,ultrasonic oscillation time of 35 min,the centrifugal speed and time respectively was 12000 r / min and 10 min,SAL and RAC preconcentration factor was42.3 and 39.5,the linear range of 10 ng / m L to 100 ng / mL. Conclusion: the analysis based on the combination of UAIS-IL-DLPM-E and HPLC has helped to concentrate,purify and quickly detect the targeted compounds RAC and SAL in feed,animal innards and muscle samples.
下载: