聚乙二醇对不同木质纤维底物酶解生产葡萄糖的影响

Effect of polyethylene glycol on enzymatic hydrolysis for glucose production with different lignocellulosic substrates

摘要: 研究了聚乙二醇(PEG)对纤维素酶水解两种不同底物产葡萄糖的影响,添加PEG后两种底物的纤维素生成葡萄糖的转化率均得到提高,PEG400浓度为9 g/L时转化率达到最大值。PEG对经酸处理的玉米秸秆的糖化促进效果(提高30%)大于经酸碱处理的玉米秸秆(提高10%)。当反应体系的固形物提高或酶加量增大时,PEG对糖化作用的促进效果降低。发酵实验结果表明PEG对酵母无不良影响。

Abstract: The effects of polyethylene glycol( PEG) on the production of glucose from two different substrates by cellulase hydrolysis were studied.The conversion of cellulose to glucose was improved after the addition of PEG to the two substrates.The conversion rate reached maximum value when PEG concentration was 9 g / L.The promoting effect of PEG on acid-treated corn straw( increased by 30%) was greater than the acid-alkali-treated corn straw( increased by 10%).The promoting effect of PEG on saccharification was reduced when the solid content of the reaction mixture was increased or the amount of the enzyme was increased.The fermentation experiment showed that PEG had no adverse effect on yeast