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中国精品科技期刊2020
李倩, 李晨阳, 买吾拉江·阿不都热衣木, 徐芳, 赵军. 高速逆流色谱法分离纯化新疆圆柏枝叶中的黄酮类成分[J]. 食品工业科技, 2016, (22): 59-61. DOI: 10.13386/j.issn1002-0306.2016.22.003
引用本文: 李倩, 李晨阳, 买吾拉江·阿不都热衣木, 徐芳, 赵军. 高速逆流色谱法分离纯化新疆圆柏枝叶中的黄酮类成分[J]. 食品工业科技, 2016, (22): 59-61. DOI: 10.13386/j.issn1002-0306.2016.22.003
LI Qian, LI Chen-yang, Maiwulajiang ABUDUREYIMU, XU Fang, ZHAO Jun. Separation and purification of flavonoids from Juniperus sabina L.by high-speed counter-current chromatography[J]. Science and Technology of Food Industry, 2016, (22): 59-61. DOI: 10.13386/j.issn1002-0306.2016.22.003
Citation: LI Qian, LI Chen-yang, Maiwulajiang ABUDUREYIMU, XU Fang, ZHAO Jun. Separation and purification of flavonoids from Juniperus sabina L.by high-speed counter-current chromatography[J]. Science and Technology of Food Industry, 2016, (22): 59-61. DOI: 10.13386/j.issn1002-0306.2016.22.003

高速逆流色谱法分离纯化新疆圆柏枝叶中的黄酮类成分

Separation and purification of flavonoids from Juniperus sabina L.by high-speed counter-current chromatography

  • 摘要: 目的:建立高速逆流色谱分离纯化新疆圆柏枝叶中的黄酮类成分的方法。方法:以氯仿∶甲醇∶正丁醇∶水(4∶3∶0.5∶2)为溶剂系统,上相为固定相,下相为流动相,在流速2.0 m L/min、转速850 r/min、检测波长254 nm的条件下,对新疆圆柏中的黄酮类成分进行分离纯化。结果:从新疆圆柏提取物中分离纯化得到3个化合物:异槲皮苷(a,91.89%)、槲皮苷(b,98.45%)、槲皮素-3-O-(6″-O-乙酰基)-β-D-吡喃葡萄糖苷(c,95.35%),其中c为首次从圆柏属植物中分离得到的化合物。结论:该法快速简便,能够高效分离新疆圆柏中的黄酮类成分。 

     

    Abstract: Objective: To study separation and purification of flavonoids from the leaves of Juniperus sabina L.by high- speed counter- current chromatography( HSCCC).Methods: Solvent system of chloroform∶ methanol∶ n- butanol∶water( 4∶ 3∶ 0.5∶ 2) was employed,the upper phase as stationary phase,the lower phase as mobile phase,a flowrate of 2.0 m L / min,the rotation speed 850 r / min and detection wavelength 254 nm,flavonoids from Juniperus sabina L.were isolated and purified under this condition.Results: Three compounds were separated from the leaves of Juniperus sabina L.as isoquercitrin( a,91.89%),quercitrin( b,98.45%),quercetin-3- O-( 6″- O- acetyl)-β- Dglucopyranoside( c,95.35%),of which compound c was separated from Juniperus genus for the first time.Conclusion: This method could conveniently and effectively separate flavonoids from Juniperus sabina L.

     

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