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中国精品科技期刊2020
吉义平, 费珊, 丁彩梅. 双酶法提取豆豉多糖的工艺研究[J]. 食品工业科技, 2016, (21): 260-264. DOI: 10.13386/j.issn1002-0306.2016.21.041
引用本文: 吉义平, 费珊, 丁彩梅. 双酶法提取豆豉多糖的工艺研究[J]. 食品工业科技, 2016, (21): 260-264. DOI: 10.13386/j.issn1002-0306.2016.21.041
JI Yi-ping, FEI Shan, DING Cai-mei. Research on dual-enzymatic extraction of polysaccharides from Douchi[J]. Science and Technology of Food Industry, 2016, (21): 260-264. DOI: 10.13386/j.issn1002-0306.2016.21.041
Citation: JI Yi-ping, FEI Shan, DING Cai-mei. Research on dual-enzymatic extraction of polysaccharides from Douchi[J]. Science and Technology of Food Industry, 2016, (21): 260-264. DOI: 10.13386/j.issn1002-0306.2016.21.041

双酶法提取豆豉多糖的工艺研究

Research on dual-enzymatic extraction of polysaccharides from Douchi

  • 摘要: 在单因素实验基础上应用正交实验设计法对双酶法提取豆豉多糖的工艺进行了优化。优化后的最佳提取工艺为:首先在料液比1∶20(g∶m L)、p H7.0和温度50℃的条件下,添加2.0%的木瓜蛋白酶进行水解150 min。然后在料液比1∶20(g∶m L)、p H7.0和55℃的条件下继续添加2.5%的风味蛋白酶水解150 min,两次分步酶解后测得多糖得率为15.90%。此优化后的豆豉多糖提取工艺合理、可行且得率较高。 

     

    Abstract: Based on the single factor experiments,the orthogonal experimental design was employed to optimize the extraction conditions of polysaccharide from douchi via dual-enzymatic.The results showed that the optimum dual-enzymatic extracting conditions were as follows: firstly,the ratio of water to raw material 1∶ 20( g ∶ m L),p H7.0and extraction temperature 50 ℃,enzyme dosage of papain 2% and hydrolysis time 150 min.Secondly,p H7.0 and extraction temperature 55 ℃,with flavourzyme 2.5%,hydrolysis time 150 min.Under these step by step enzymatic hydrolysis conditions,the extraction yield was 15.90%. The optimization of extraction process technology of polysaccharides from douchi was reasonable and feasible and yield was higher.

     

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