Abstract: To investigate the biological characteristics of a Lactobacillus rhamnosus strain with anti-diabetic activity.The results showed that the logarithmic time of this Lactobacillus rhamnosus strain was from 2th hour to 14 th hour.The optimum p H and temperature for its growth was 5 ~ 7 and 37 ℃,respectively,and its optimum inoculated concentration was 1% ~2%( V / V). Besides,this strain could grow under strongly acidic conditions,and survive in the simulated gastro-intestinal juice.The bile tolerance of this strain in the form of lag time was 0.86 h,and it could grow under 9% Na Cl( W/ V) conditions.More importantly,it possessed strong adhesive properties to Caco-2 cells.In summary,this Lactobacillus rhamnosus strain with antidiabetic activity could be applied to develop novel fermented food products with hypoglycemic effect.