Abstract: The aim of this study was to survey and analyze the antibacterial resistance of Vibrio parahaemolyticus,which were isolated from the commercial aquatic products in Shanghai markets.45 V.parahaemolyticus strains were isolated in two years,and all the isolates were carried out for 25 kinds of antibiotic susceptibility pattern using modified Kirby- Bauer disk diffusion method.The results showed that all strains were resistance to Penicillin G and Metronidazole.More than 90% of the V. parahaemolyticus isolates were resistant to Piperacillin,Neomycin and Kanamycin,and all strains were sensitive to Rifampicin,Vancomyci,Tetracycline,Doxycycline,Cephradine and Oxytetracycline.Moreover,antibiotic resistance patterns of the strains existed a certain differences among isolation origin.And there was a certain relation between the multi- drug resistance and pathogenic types of the strains.For this reason, it is important to take measures to strengthen monitor the antibacterial resistance of V.parahaemolyticus in the contaminated aquatic products.