Abstract: To investigate the conditions of nanoparticles formation of carp egg peptide( CEP) induced by Ca²⁺and p H and to demonstrate the force of stabling their space structures. The conditions could be preliminary screened by turbidity testing and confirmed by particle size analysis and electron microscope observation. The forces of stabling nanoparticles could be determined through secondary bond destruction experiments and the changes of their space structures were studied using Fourier transform infrared spectroscopy( FTIR) and circular dichroism spectroscopy( CD).The results showed that nanoparticles whose diameters ranging from 20~100 nm were formed by CEP and Ca²⁺under p H6.0~9.0.The stabilities of the nanoparticles were mainly dependent on hydrophobic force and electrostatic force.The β- sheet formation was observed while CEP formed nanoparticles.CEP could be used as a biological template to synthesize peptide- calcium nanoparticles under mild conditions.