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中国精品科技期刊2020
张珉, 孙化鹏, 唐效蓉, 黄帆, 夏合新, 许忠坤. 星点设计-效应面法优化马尾松花粉总黄酮提取工艺[J]. 食品工业科技, 2016, (15): 230-233. DOI: 10.13386/j.issn1002-0306.2016.15.036
引用本文: 张珉, 孙化鹏, 唐效蓉, 黄帆, 夏合新, 许忠坤. 星点设计-效应面法优化马尾松花粉总黄酮提取工艺[J]. 食品工业科技, 2016, (15): 230-233. DOI: 10.13386/j.issn1002-0306.2016.15.036
ZHANG Min, SUN Hua-peng, TANG Xiao-rong, HUANG Fan, XIA He-xin, XU Zhong-kun. Optimization of extraction process of flavone from the Pinus massoniana pollen by using central composite design- response surface methodology[J]. Science and Technology of Food Industry, 2016, (15): 230-233. DOI: 10.13386/j.issn1002-0306.2016.15.036
Citation: ZHANG Min, SUN Hua-peng, TANG Xiao-rong, HUANG Fan, XIA He-xin, XU Zhong-kun. Optimization of extraction process of flavone from the Pinus massoniana pollen by using central composite design- response surface methodology[J]. Science and Technology of Food Industry, 2016, (15): 230-233. DOI: 10.13386/j.issn1002-0306.2016.15.036

星点设计-效应面法优化马尾松花粉总黄酮提取工艺

Optimization of extraction process of flavone from the Pinus massoniana pollen by using central composite design- response surface methodology

  • 摘要: 采用超声辅助提取法从马尾松花粉中提取总黄酮,通过单因素实验考察乙醇体积分数、提取时间、料液比以及提取次数等4个因素对马尾松花粉总黄酮得率的影响,并采用星点设计-效应面法优化总黄酮提取工艺。结果表明:马尾松花粉总黄酮的最佳提取工艺条件为乙醇体积分数62.5%,提取时间28.8 min,料液比1∶24,提取次数3次。在此优化条件下,马尾松花粉总黄酮的实际得率为1.37%,与理论值(1.32%)较为接近,表明采用星点设计-效应面法优化马尾松花粉总黄酮提取工艺的方法可行。 

     

    Abstract: The ultrasound- assisted extraction of flavone from the Pinus massoniana pollen was carried out. The effects of ethanol concentration,extraction time,material- liquid ratio and extraction numbers on the extraction rate of flavone were studied by single factor experiment,and the extraction process of flavone was optimized by the central composite design- response surface methodology. The results showed that,the optimal extraction conditions of flavone from the Pinus massoniana pollen were 62.5% of ethanol concentration,28.8 minutes of extraction time,1 ∶ 24 of material- liquid ratio and 3 times of extraction number. Under this optimal condition,the actual extraction rate of flavone reached 1.37%,which was close to the theoretic value.It was feasible to optimize the extraction process of flavone from the Pinus massoniana pollen by using central composite design- response surface methodology.

     

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