响应面法优化甲烷氧化菌3011高产甲烷氧化菌素的研究

Application of response surface methodology on high yield methanobactin by Methylosinus trichosporium IMV 3011

摘要: 利用响应面方法对甲烷氧化菌3011发酵产甲烷氧化菌素（Mb）条件进行了优化。以Mb产量为考察指标,利用Plackett-Burman设计对影响产量的7个因素进行评价,筛选出具有显著效应的3个因素,这三个因素分别为甲醇流加量、铜离子浓度和p H。利用Box-Behnken实验设计对显著因素进行优化,得到产Mb的最优发酵培养条件为甲醇流加量54.1μmol/min,p H6.39,铜17.03μmol/L,温度30℃,接种量5×10⁶CFU/m L,通氧量30 L/h,搅拌转速400 r/min,此条件下Mb产量为3300.2μg/m L。由此可得响应面优化得到的生产工艺是提高Mb产量的可行方法。

Abstract: To optimize Methanobactin( Mb) fermentation conditions for Methylosinus trichosporium 3011,Response Surface Methodology( RSM) were applied. The methanol flow,copper ion content and p H of seven factors were determined by Plackett- Burman design. The optimum condition was as follows: the methanol flow rate of54.1 μmol / min,the p H of 6.39,the copper content of 17.03 μmol / L,the incubation temperature of 30 ℃,the inoculation amount of 5 × 10~6 CFU / m L,the dissolved oxygen of 30 L / h,the stirring speed of 400 r / min.The optimum Mb production was 3300.2 μg / L. The RSM was feasible for high Mb production and this would improve the Mb production.