Abstract: With aim of developing a speed,accurate,one- step enzyme- linked immunosorbent assay( ELISA) for the detection of zearalenone in feed and feed stuffs was set. Hapten was synthesized firstly,conjugated then to carrier proteins for complete antigen,which was used as antigen in mice for monoclonal antibody.Concentrations of coating antigen and antibody were determined with chessboard format.A stable analytical system was established and the specificity of monoclonal antibody was evaluated. The results showed that the detection of limit of this developed ELISA for zearalenone in pig feed、cow feed、maize pulp and wheat bran was 96.2,92.7,80.5 and86.7 μg / kg,respectively.Feed samples were spiked with zearalenone and the recoveries of ELISA were 79.4% ~104.9%.There was no distinct difference between the results of WDWK and RIDA kits. This study could bring a technical basis for the monitor of zearalenone.