Abstract: In order to further understand the rule of heat treatment on postharvest fruits and vegetables in the metabolism,looking for optimum heat treatment temperature of melon on the metabolism. Melon was used in this study to reveal effects of metabolic network flux at heat treatment. The melon was placed in 45,50 and 55 ℃for three hours,then placed hami melon immediately in optimal storage temperature 8 ℃ under 21 hours,as a blank to 8 ℃ for 24 hours,the metabolic flux of embden meyerhof pathway(EMP),pentose phosphate pathway(PPP),TCA cycle(TCA) and sucrose synthesis pathway(SSP) of hami melon fruit different spatial location at different temperatures were researched. The results showed that higher sugar and lactate flux appeared in the heat treatment group of 50 ℃. Thus,50 ℃ was the optimal heat treatment temperature. This result combined with early work,to summarize the influence of heat treatment on metabolism,with a view to provide a scientific method and theory on the heat treatment to improve the quality of fruits and vegetables.