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中国精品科技期刊2020
张占军, 杨小生. 响应面法优化土党参多糖的提取工艺及其神经营养活性研究[J]. 食品工业科技, 2016, (06): 291-297. DOI: 10.13386/j.issn1002-0306.2016.06.051
引用本文: 张占军, 杨小生. 响应面法优化土党参多糖的提取工艺及其神经营养活性研究[J]. 食品工业科技, 2016, (06): 291-297. DOI: 10.13386/j.issn1002-0306.2016.06.051
ZHANG Zhan-jun, YANG Xiao-sheng. Optimization of extraction conditions of polysaccharides from Campanumoea javanica Blume by response surface methodology and evaluation of its neurotrophic activities[J]. Science and Technology of Food Industry, 2016, (06): 291-297. DOI: 10.13386/j.issn1002-0306.2016.06.051
Citation: ZHANG Zhan-jun, YANG Xiao-sheng. Optimization of extraction conditions of polysaccharides from Campanumoea javanica Blume by response surface methodology and evaluation of its neurotrophic activities[J]. Science and Technology of Food Industry, 2016, (06): 291-297. DOI: 10.13386/j.issn1002-0306.2016.06.051

响应面法优化土党参多糖的提取工艺及其神经营养活性研究

Optimization of extraction conditions of polysaccharides from Campanumoea javanica Blume by response surface methodology and evaluation of its neurotrophic activities

  • 摘要: 对土党参多糖(CJP)的提取条件,分离纯化及其理化性质进行了研究,并研究了土党参多糖对大鼠嗜铬细胞瘤PC12细胞的分化作用。通过单因素实验及响应面优化实验,土党参多糖最佳提取条件确定为提取温度67℃、提取时间52 min和液料比17∶1 m L/g。通过DEAE-52纤维素柱层析分离,葡聚糖凝胶Sephadex G-100柱层析纯化,得到两种土党参多糖CJPH和CJPN。采用化学分析方法分析了土党参多糖的总糖、蛋白质、糖醛酸及硫酸基含量。通过高效液相凝胶渗透色谱法进行纯度鉴定和相对分子质量测定表明,CJPH和CJPN为均一多糖,其重均分子量Mw分别为3923 u和7215 u。通过气相色谱法对其单糖组成进行了分析,采用红外光谱研究了其结构特征。通过PC12细胞培养表明,CJPH和CJPN均具有神经营养活性。研究表明,制备的土党参多糖能明显修复损伤的神经细胞,具有一定的开发利用价值。 

     

    Abstract: Extraction optimization, purification and physicochemical properties of polysaccharides from Campanumoea javanica Blume( CJP) were investigated. The neurotrophic activity of polysaccharides from C. javanica Blume were investigated by PC12 cell assay. Single factor experiment and response surface methodology(RSM) were used to optimize the extraction conditions of CJP. As a result,the optimal conditions were as follows : extraction temperature, 67 ℃, extraction time, 52 min, and ratio of water to raw material,17 ∶ 1 m L / g. The crude CJP were purified by DEAE- Cellulose 52 chromatography and Sephadex G- 100 chromatography to afford CJPH and CJPN. The content of total sugar,protein,uronic acid and sulfate were analyzed by chemical analysis methods. HPLC analysis showed that CJPH and CJPN were homogeneous polysaccharide,the molecular weights of CJPH and CJPN were 3923 u and 7215 u,respectively. Monosaccharide composition analysis was acted by using the GC method. Infrared spectroscopy analysis indicated that CJPH and CJPN showed typical peaks of polysaccharide. The assay of PC12 cell showed that CJPH and CJPN had neurotrophic activity. Studies had shown that CJPH and CJPN could obviously repairing nerve cell damage,they had certain development value.

     

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