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中国精品科技期刊2020
阳晖, 赵学勤, 李昌满, 孙钟雷, 李宇, 李敏, 邓鑫, 胡苇. 胭脂萝卜废渣中提取萝卜硫素的酶解工艺优化[J]. 食品工业科技, 2016, (05): 207-211. DOI: 10.13386/j.issn1002-0306.2016.05.032
引用本文: 阳晖, 赵学勤, 李昌满, 孙钟雷, 李宇, 李敏, 邓鑫, 胡苇. 胭脂萝卜废渣中提取萝卜硫素的酶解工艺优化[J]. 食品工业科技, 2016, (05): 207-211. DOI: 10.13386/j.issn1002-0306.2016.05.032
YANG Hui, ZHAO Xue- qin, LI Chang- man, SUN Zhong- lei, LI Yu, LI Min, DENG Xin, HU Wei. Optimization of enzymatic hydrolysis conditions for extracting sulforaphane from waste of carmine radish[J]. Science and Technology of Food Industry, 2016, (05): 207-211. DOI: 10.13386/j.issn1002-0306.2016.05.032
Citation: YANG Hui, ZHAO Xue- qin, LI Chang- man, SUN Zhong- lei, LI Yu, LI Min, DENG Xin, HU Wei. Optimization of enzymatic hydrolysis conditions for extracting sulforaphane from waste of carmine radish[J]. Science and Technology of Food Industry, 2016, (05): 207-211. DOI: 10.13386/j.issn1002-0306.2016.05.032

胭脂萝卜废渣中提取萝卜硫素的酶解工艺优化

Optimization of enzymatic hydrolysis conditions for extracting sulforaphane from waste of carmine radish

  • 摘要: 以胭脂萝卜废渣为原料,提取萝卜硫素。为提高萝卜硫素的得率,优化了胭脂萝卜废渣中萝卜硫苷的酶解条件。在单因素实验的基础上,以响应面分析法分别探讨了四个主要影响因素(酶解温度、酶解时间、p H、抗坏血酸(VC)添加量)对胭脂萝卜废渣中萝卜硫素得率的影响,并进一步优化了硫代葡萄糖苷酶解条件。结果表明:在酶解温度为31℃,酶解时间为7.7 h,p H5.6,VC添加量为0.24 mg/kg条件下,萝卜硫素的得率最高为0.05613%,其结果与模型预测值相符。这说明采用响应面法优化获得的提取条件较可靠。 

     

    Abstract: In this study,sulforaphane was extracted from the waste of carmine radish.In order to enhance the yield of sulforaphane,enzymatic hydrolysis conditions of glucosinolates in waste of carmine radish were optimized.Based on single- factor experiments,the effects of four factors( the hydrolysis temperature,hydrolysis time,amount of VC,p H value) on the yield of sulforaphane were investigated by response surface methodology.Experimental results showed that,when the hydrolysis temperature was 31 ℃,hydrolysis time was 7.7 h,amount of VCwas0.24 mg / kg,p H was 5.6,the yield of sulforaphane was the highest with the value of 0.05613%,which were according with the predicted value of model.This indicated that extraction conditions which analyzed by response surface method were more reliable.

     

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