Abstract:
In this study,sulforaphane was extracted from the waste of carmine radish.In order to enhance the yield of sulforaphane,enzymatic hydrolysis conditions of glucosinolates in waste of carmine radish were optimized.Based on single- factor experiments,the effects of four factors( the hydrolysis temperature,hydrolysis time,amount of VC,p H value) on the yield of sulforaphane were investigated by response surface methodology.Experimental results showed that,when the hydrolysis temperature was 31 ℃,hydrolysis time was 7.7 h,amount of VCwas0.24 mg / kg,p H was 5.6,the yield of sulforaphane was the highest with the value of 0.05613%,which were according with the predicted value of model.This indicated that extraction conditions which analyzed by response surface method were more reliable.