Abstract: Objective:To discuss the influence of beer qualities brewed with malts mixed by wheat and barley in different ratios by measuring the changes of chemical materials in brewing process. Methods:The common chemical methods,such as Congo red method,high efficiency liquid chromatography and gas chromatography were used to measure the content of β-glucans,araboxylan,organic acid and volatile matter in beer. Results:Adding the wheat malt mainly lead that the viscosity,esters contents and most of organic acid were increased.The alcohol was a liner relation with wheat malt added ratios. When the wheat malt ratio was 50%,wheat beer had the best sensory evaluation. Conclusions:The quality of beer was enhanced obviously measured by the changes of chemical content when wheat mixed in barley malt.