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中国精品科技期刊2020
徐顾榕, 蔡婷, 林凯, 宋菲菲, 张庆, 向文良. 四川泡豇豆原料表皮附生乳酸菌抗生素抗性及抗性基因污染分析[J]. 食品工业科技, 2016, (02): 170-175. DOI: 10.13386/j.issn1002-0306.2016.02.026
引用本文: 徐顾榕, 蔡婷, 林凯, 宋菲菲, 张庆, 向文良. 四川泡豇豆原料表皮附生乳酸菌抗生素抗性及抗性基因污染分析[J]. 食品工业科技, 2016, (02): 170-175. DOI: 10.13386/j.issn1002-0306.2016.02.026
XU Gu-rong, CAI Ting, LIN Kai, SONG Fei-fei, ZHANG Qing, XIANG Wen-liang. Investigation of antibiotic resistance and resistant genes of epibiotic lactic acid bacteria on the surface of Sichuan pickled cowpea[J]. Science and Technology of Food Industry, 2016, (02): 170-175. DOI: 10.13386/j.issn1002-0306.2016.02.026
Citation: XU Gu-rong, CAI Ting, LIN Kai, SONG Fei-fei, ZHANG Qing, XIANG Wen-liang. Investigation of antibiotic resistance and resistant genes of epibiotic lactic acid bacteria on the surface of Sichuan pickled cowpea[J]. Science and Technology of Food Industry, 2016, (02): 170-175. DOI: 10.13386/j.issn1002-0306.2016.02.026

四川泡豇豆原料表皮附生乳酸菌抗生素抗性及抗性基因污染分析

Investigation of antibiotic resistance and resistant genes of epibiotic lactic acid bacteria on the surface of Sichuan pickled cowpea

  • 摘要: 以鲜豇豆为研究对象,利用生理生化特征、16S r RNA、抗生素抗性和抗性基因检测分析其表皮附生乳酸菌对氯霉素(CHL)、四环素(TET)和氨苄青霉素(AMP)的抗性与抗性基因。结果表明:从鲜豇豆表皮共分离得到182株乳酸菌,分别属于Weissella confuse(50.0%)、Weissella cibaria(15.4%)、Enterococcus sulfurous(7.1%)和Lactococcus lactis subsp.lactis(27.5%)。32株(17.6%)乳酸菌对CHL和TET有抗性,其中,6株W.confuse(3.3%)和4株L.lactis subsp.Lactis(2.1%)对CHL单一抗性,4株W.confuse(2.1%)和5株L.lactis subsp.Lactis(2.7%)对TET单一抗性,6株W.confuse(3.2%)、2株W.cibaria(1.1%)、1株E.sulfurous(0.5%)和4株L.lactis subsp.Lactis(2.1%)对CHL和TET二重抗性;编码外排泵基因efr A、efr B、nor B、nor C、nor E和sug E中,efr B的检出率最高,达31.3%,efr B和sug E的检出率最低,为3.1%。TET被检抗性基因tet(A)、tet(B)、tet(C)、tet(D)、tet(H)和tet(L)中,tet(C)的检出率最高,达90.9%,tet(B)的检出率最低,为4.5%。 

     

    Abstract: The epibiotic lactic acid bacteria(LABs) on the fresh cowpea,which resist to chloramphenicol(CHL)tetracycline(TET) and ampicillin(AMP),were investigated by the physiological and biochemical characteristics,16 S r RNA,antibiotic resistance and resistant genes. 182 isolates in current study were assigned to Weissella confuse(50.0%),Weissella cibaria(15.4%),Enterococcus sulfurous(7.1%) and Lactococcus lactis subsp. lactis( 27. 5 %), respectively. And 32 isolates( 17. 6 %) were found to be against CHL and TET with one or two resistance,including 6 W. confuse(3.3%) and 4 L. lactis subsp. lactis(2.1%) with resistance to CHL,and 4W. confuse(2.1%) and 5 L. lactis subsp. lactis(2.7%) with resistance to TET. 6 W. confuse(3.2%),2 W. cibaria(1.1%),1 E. sulfurous(0.5%) and 4 L. lactis subsp. lactis(2.1%) with resistances to CHL and TET. In the effux pump genes efr A,efr B,nor B,nor C,nor E and sug E,efr B had the highest detection rate with 31.3%,but efr B and sug E with only 3.1%. Among the TET resistant genes tet(A),tet(B),tet(C),tet(D),tet(H) and tet(L),tet(C)had the highest detection rate,90.9%,and tet(B) was found with 4.5% lowest detection rate.

     

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