Abstract: A new kind of beverage was prepared through linden tree honey as materials,and Saccharomycodes ludwigii,Gluconacetobacter and Lactobacillus acidophilus were used to carry on symbiotic fermentation of multiple strains. Mice were divided randomly into 5 groups:control group,high fat nutritional control group,honey vinegar prevention of overweight,hyperglycemia model,and honey vinegar antilipemic group. Each group except the control group was dosed once a day for 30 days and was weighed once every 5 days. The body weight,body fat,serum total cholesterol(TC),triglyceride(TG) and high-density lipoprotein cholesterol(HDL-C) of the mice were determined. The results of test showed that the honey fermented beverage could obviously all reduce the weight of adiposity mice(p <0.05),fat index number(p <0.05),TG(p <0.05),TC(p <0.05),debasing AI. Yet improve their HDL in blood serum,so the honey fermented beverage possesses great functions in reducing weight,fat and preventing arteriosclerosis.