西洋参配方颗粒质量标准及指纹图谱的研究
Study on quality standard and fingerprint of American ginseng granule
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摘要: 目的:建立西洋参配方颗粒质量控制方法。方法:通过TLC对西洋参进行鉴别,用不同产地的10批西洋参制成配方颗粒,建立HPLC指纹图谱。色谱条件为,色谱柱:ZORBAX SB-C18(250 mm×4.6 mm,5μm);流动相:乙腈-0.1%磷酸溶液梯度洗脱;检测波长:203 nm;柱温:25℃。结果:薄层色谱鉴别方法专属性强,斑点清晰,且阴性无干扰;人参皂苷Rg1、人参皂苷Re及人参皂苷Rb1分别在0.07940.4764μg/m L(r=1)、0.3181.908μg/m L(r=0.9998)、0.82164.9296μg/m L(r=0.9999)范围内线性关系良好。建立了西洋参的指纹图谱,确定13个共有峰,相似度在0.9440.988之间。结论:实验方法结果准确、重复性好。HPLC指纹图谱的建立,能够对该配方颗粒的鉴别与质量评价做出较全面的反映。Abstract: Objective:To establish the quality control method of American ginseng granule. Methods:10 batches of American ginseng from different regions were identified by TLC and made into American ginseng granules which were established quality standard by HPLC fingerprint. The HPLC condition was that:ZORBAX SB-C18(250 mm×4.6 mm,5 μm) column,gradient elution of mobile phase of acetonitrile-0.1% phosphoric acid solution,203 nm detection wavelength,25 ℃ column temperature. Results:The TLC method showed well specificity,clear spots with no negative interference. The fine linear ranges of Ginsenoside Rg1,Re and Rb1 were 0.0794~0.4764 μg/m L(r =1),0.318 ~1.908 μg/m L(r =0.9998),0.8216 ~4.9296 μg/m L(r =0.9999) respectively. The fingerprint of American ginseng was established with 13 common peaks and a similarity between 0.944 to 0.988.Conclusion:The method was accurate with good reproducibility. HPLC fingerprint provided a comprehensive reflection on identification and quality evaluation of the granule.