孜然精油对杂拟谷盗的熏蒸效果及代谢酶活的影响
Control with essential oils of cumin(Cuminum cyminum) against Tribolium confusum and influence of metabolic enzyme activity
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摘要: 本文研究了孜然精油对杂拟谷盗（Tribolium confusum）的熏蒸效果及部分机理。T.confusum经最大剂量30μL/L孜然精油处理24 h后,死亡率达到了95%,试虫的死亡率与处理浓度和处理时间呈现出明显的正相关。T.confusum经孜然精油熏蒸处理后乙酰胆碱酯酶（Ach E）在8 h出现最高值,解毒酶系中的酸性磷酸酯酶（ACP）呈现先下降后升高的趋势;碱性磷酸酯酶（ALP）、羧酸酯酶（Car E）活性明显高于未熏蒸的对照,且这两种酶活力均呈现出升高的趋势;谷胱甘肽-S-转移酶（GSTs）的酶活性被显著（p≤0.05）地抑制;保护酶系中的三种酶活性也被精油的处理诱导升高。由此表明,孜然精油对T.confusum的良好熏蒸效果是通过影响试虫体内的神经、解毒和保护酶系来实现的。Abstract: The study was used the methods of fumigant to measure the effects of physiological and biochemical index of T. confusum. The motality of T. confusum was 95% after 24 h of treatment at 30 μL/L cumin essential oil. The motality rate showed positive correlation with concentration and treatment time. Through T. confusum fumigant by the essential oil of cumin,the result showed that the activity of Ach E was appeared highest at 8 h,detoxification enzymes ACP presented variation trend,increased at first and then decreased. ALP and Car E activity were significantly higher than the control fumigation and vitality of these two enzymes were shown a rising trend. The activity of GST was significant inhibited(p ≤0.05) during the treatment. In conclusion,the essential oil of cumin possessed activity of repellency and fumigation,and contact toxicity against T. confusum.The insectieidal mechanism mainly involved the activities of many impotant enzymes.
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