Abstract: The induced autolytic wine yeast cell wall was used as raw materials to extract β-glucan. On the basis of single factor test including solid- liquid ratio, alkali concentration, extraction time and extraction temperature,the orthogonal test was applied to determine the optimal extraction conditions. The results showed that the optimum conditions of β-glucan were as following:solid-liquid ratio 1∶40(g/m L),the concentration of Na OH 3%,extraction temperature 80 ℃ and extraction time 1.5 h. Under these conditions,the yield of β-glucan was 19.38%. The process was simple and inexpensive and had higher extraction rate.