Abstract: This study aimed to clone pig β2AR gene,analysize its sequence and construct its recombinant yeast expression plasmid. Total RNA was extracted from pig liver, and then RT- PCR was conducted based on published pig β2AR gene sequence(AF000134). By cloning of the PCR product and identification,the c DNA fragment of pig β2AR was obtained. Bioinformatical tools were adopted to analyze the gene sequence and amino acid. The target gene was cloned to p PICZα A vector,constructing recombinant expression plasmid.The c DNA fragment of pig β2AR(KF023571.1) was 1257 bp,and encodeed 418 amino acids. The deduced protein was predicted to have a computed molecular mass of 46.73 ku. There were high similarity(more than83%) and homology in β2AR gene between pig and other species. Finally,recombinant plasmid named p PICZαA- β2AR was constructed successfully after verification by PCR, restriction enzyme analysis and sequencing. This research will serve as a base for expression of pig β2AR gene in yeast expression system and its application in multiresidue determination of β2-adrenoceptor agonists.