Abstract: The enzymolysis process and anti- oxidation activity in vitro of antioxidant peptides extracted from apricot kernel were studied in this paper. Taking hydroxyl radical scavenging rate and degree of hydrolysis( DH) as indexes,effect of enzyme dosage,substrate concentration,temperature and p H on the enzymolysis process of apricot kernel antioxidant peptides with response surface analysis were studied. The optimum conditions were adding amount of compound enzyme from alkaline proteinase and flavourzyme 6725 U / g( the dosages of alkaline proteinase and flavourzyme were 3645 U / g and 2690 U / g,respectively),substrate mass fraction 3%,temperature60 ℃,p H8.28.Under these conditions,the scavenging rate and DH were respectively 84.18% and 20.87%.