Abstract: The oil was extracted from ditrema offal by high voltage pulsed electric field. Extraction yield was optimized by response surface methodology(RSM) on the basis of a single factor. The effect of electric field intensity,pulse frequency,extraction time and liquid-solid ratio on extraction yield of oil were investigated. The optimum extraction process conditions were as follows:the electric field intensity 18 k V·cm-1,pulse frequency300 Hz,extraction time 65 min and liquid-solid ratio 1∶1. Under those,the actual extraction yield of oil was15.18%. The quadratic polynomial regression model was obtained by the regression equation fitting and response surface analysis. The predictive and experimental results were found to be in good agreement. Thus,the model was applicable for the prediction of the extraction yield of oil from ditrema offal.