鲟龙鱼硫酸软骨素提取工艺优化研究

Optimization technology of extraction of the chondroitin sulfate from sturgeon

摘要: 利用响应曲面法优化提取西伯利亚鲟龙鱼软骨组织提取硫酸软骨素的工艺条件。研究了碱浓度、沉淀时间、乙醇体积对硫酸软骨素提取率的影响,在单因素实验基础上采用Box-Behnken中心组合实验,以碱质量浓度、沉淀时间、乙醇体积为影响因素,以提取率为响应值建立二元多次方程,通过响应面分析法得到优化组合。结果表明,最佳工艺条件为:Na OH 0.5mol/L、乙醇体积1倍、沉淀时间20min,提取得硫酸软骨素产率为57.21%,预测值产率为59.31%。

Abstract: Extraction of the chondroitin sulfate in sturgeon was studied by response surface methodology. The influence of alkalinity, sedimentation time, ethanol volume on extract rate of chondroitin sulfate was studied.On the basis of single- factor investigation, Box- Benhnken central composite experiments were carried out to build the quadratic regression model for the extraction yield with above 3 factors, the optimizing process parameters were:alkalinity 0.5mol / L, sedimentation time 20 min, ethanol volume 1 times of the extraction solution. Under the optimal processing condition, the yield of chondroitin sulfate was 57.21%, and the forecasted yield was 59.31%.