Abstract: Amino acid composition of puerarin spirulina compound tablets was determined by automatic amino acid analyzer. Protein nutritional value of amino acid composition of puerarin spirulina compound tablets was carried on the comprehensive analysis and evaluation to the egg protein as standard protein, by WHO/FAO reference model for the analysis and evaluation standard by the amino acid ratio coefficient method and fuzzy recognition method. Taking mouse as experimental material, the preliminary experiment of anti-inebriation of puerarin spirulina compound tablets was studied with mouse drunken behavior observation ( drunken and sober) as an index. The results showed that puerarin spirulina compound tablets contained 18 kinds of amino acid, total content of 18 kinds of amino acid was 32.46%. Type amino acids in the protein was complete.Essential amino acid (EAA) accounted for 37.22% of total amino acid content. The first limiting amino acid was tryptophan. The second limiting amino acid was sulfur amino acids. Amino acid ratio coefficient was divided into 75.18 ⁸2.41. Puerarin spirulina compound tablets to ethanol acute poisoning had significant preventive effect ( p < 0. 01) . Puerarin spirulina compound tablets had a more significant role in solution wine decanter (p <0.05) . Puerarin spirulina compound tablets was better than the solution wine decanter prevention drunk effect (p<0.01) .