Abstract: In this paper, pre- column derivatization HPLC was used to seperate seven kinds of biogenic amines including tyramine ( Tyr) , putrescine ( Put) , cadaverine ( Cad) , histamine ( His) , tryptamine ( Try) , spermine ( Spd) and spermidine ( Spm) in bay scallop in 20 min. And the change of biogenic amines amount in scallops was determined and analyzed duiring the storage at 0, - 5 and- 20℃. The results showed that the effect of storage temperature on the content of Spd, Spm and Cad in the scallop was significant ( p < 0.05) , and the higher the temperature was, the faster the three kinds of biogenic amines were produced, so Cad, Spm and Spd can be the reference indexes for the change of quality of scallops during storage.And freezing- point storage combined with complex biological preservatives had significant inhibitory effect on the producing of Cad, Spd and Spm in the scallop column.Therefore, freezing- point storage combined with complex biological preservatives was the effective measure of controlling the quality of scallops.