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中国精品科技期刊2020
李欣, 乔家驹, 冯汉青, 张敏, 关随霞, 刘少阳, 张素玲. 杜仲雄花乙酸乙酯提取物的抑菌活性研究[J]. 食品工业科技, 2015, (11): 62-65. DOI: 10.13386/j.issn1002-0306.2015.11.004
引用本文: 李欣, 乔家驹, 冯汉青, 张敏, 关随霞, 刘少阳, 张素玲. 杜仲雄花乙酸乙酯提取物的抑菌活性研究[J]. 食品工业科技, 2015, (11): 62-65. DOI: 10.13386/j.issn1002-0306.2015.11.004
LI Xin, QIAO Jia-ju, FENG Han-qing, ZHANG Min, GUAN Sui-xia, LIU Shao-yang, ZHANG Su-ling. Study on antibacterial activities of the ethyl acetate extract in male Eucommia flowers[J]. Science and Technology of Food Industry, 2015, (11): 62-65. DOI: 10.13386/j.issn1002-0306.2015.11.004
Citation: LI Xin, QIAO Jia-ju, FENG Han-qing, ZHANG Min, GUAN Sui-xia, LIU Shao-yang, ZHANG Su-ling. Study on antibacterial activities of the ethyl acetate extract in male Eucommia flowers[J]. Science and Technology of Food Industry, 2015, (11): 62-65. DOI: 10.13386/j.issn1002-0306.2015.11.004

杜仲雄花乙酸乙酯提取物的抑菌活性研究

Study on antibacterial activities of the ethyl acetate extract in male Eucommia flowers

  • 摘要: 本实验以杜仲雄花为材料,通过打孔法和倍比稀释法,探讨杜仲雄花乙酸乙酯提取物及经过硅胶柱分离得到的六种不同极性提取物对真菌和细菌的抑菌活性,并确定最小抑菌浓度(MIC)。结果显示:杜仲雄花乙酸乙酯提取物对供试金黄色葡萄球菌、炭疽杆菌、枯草芽孢杆菌、大肠杆菌和黑曲霉均有较好的抑制效果,最小抑菌浓度(MIC)分别为0.0156、0.0156、0.0313、0.0313、0.1250g/m L,对根霉和青霉没有抑制作用。而分离得到的六种提取物按极性不同分别为10%甲醇洗脱所得样品、20%甲醇洗脱所得样品、30%甲醇洗脱所得样品、纯甲醇洗脱所得样品、纯甲醇含氨洗脱所得样品和水洗脱所得样品,其中10%甲醇洗脱所得样品、20%甲醇洗脱所得样品与乙酸乙酯提取物活性相似或有增强,30%甲醇洗脱所得样品、纯甲醇洗脱所得样品对部分细菌有抑制作用,而只有水洗脱所得样品对根霉有抑菌作用。说明杜仲雄花乙酸乙酯提取物和进一步的硅胶柱分离得到的不同极性提取物具有良好的抑菌活性,且硅胶柱的分离能得到更佳的抑菌活性提取物,为杜仲雄花的进一步开发提供依据。 

     

    Abstract: In this study, extract of male Eucommia flowers was obtained by ethyl acetate.Six different polar extracts were further isolated from ethyl acetate extract of Eucommia by silica gel column chromatography.This study was conducted to investigate the antibacterial activities of these seven extract by using punch method or dilution method.Minimal inhibitory concentration ( MIC) of extracts was also determined. Results showed that ethyl acetate extract had good antibacterial activities against Staphylococcus aureus, Bacillus anthracis, Bacillus subtilis, Escherichia coli and Aspergillusniger. The MIC was 0.0156, 0.0156, 0.0313, 0.0313, 0.1250 g / m L, respectively. No antibacterial effect was observed on Rhizopus or Pseudomonas sp. The six compounds were named sample obtained by 10% methanol, sample obtained by 20% methanol, sample obtained by 30% methanol, sample obtained by pure methanol, sample obtained by pure methanol with a few ammonia, and sample obtained by water according to their polarities isolated by silica gel column chromatography.The activities of sample obtained by 10% methanol and sample obtained by 20% methanol were higher than that of ethyl acetate extract.While, the sample obtained by 30% methanol and sample obtained by pure methanol did not show antibacterial activities on most of strains.Strikingly, the sample obtained by water exhibited antibacterial activities on Pseudomonas sp only.Thus, the ethyl acetate extract and six compounds isolated by silica gel column chromatography had good antimicrobial activities.Silica gel column chromatography provided good separation of active extract.This research was profitable for the study and use of the male Eucommia flowers.

     

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