Abstract: The technology of separating linoleic acid from walnut oil by urea adduction was optimized in the paper. On the basis of single factor experiments, the effects of adduction temperature (℃) , adduction time (h) , m1 (mixed fatty acid) ∶m2 (urea) , V (95% ethanol) ∶m2 (urea) on the purity and yield of linoleic acid were analyzed by response surface method. The results showed that the optimal parameters of the urea adduction technology were adduction temperature-10 ℃, adduction time 24 h, m1 ( mixed fatty acid) ∶ m2 ( urea) =0. 3 and V ( 95%ethanol) ∶m2 ( urea) =4. On this condition, the purity of linoleic acid could rise from original 52.1 % to above80.20% by initial urea adduction.