硝酸银-硅胶柱层析法分离纯化核桃油中亚麻酸工艺研究

Silver nitrate-silica gel column chromatography purification linolenic acid in the walnut oil

摘要: 先采用尿素包合法对核桃油中脂肪酸进行初步分离,在此基础上利用硝酸银-硅胶柱层析法对核桃油中亚麻酸进一步纯化,20g硅胶中与2g Ag NO3配制成硝酸银硅胶柱中,加入与吸附剂比例为1∶15的经尿素包合后富集得到的脂肪酸,洗脱剂流速为0.6m L/min,丙酮-石油醚浓度为8%时,核桃油中亚麻酸由原来的8.7%洗脱后提高到95.9%,以上结果表明硝酸银-硅胶柱层析法是一种高效分离亚麻酸的方法。

Abstract: The fatty acids of walnut oil were separated primarily by urea adduction method, then the linolenic acid of walnut oil was further purified by silver nitrate-silica gel column chromatography. In the silver silica gel column formed by 20 g silica gel and 2g Ag NO3, join in the ratio was 1∶15 with adsorbent after urea inclusion enrichment of fatty acids, 100 m L 8% acetone-petroleum, underd the elution flow rate of 0.6m L/min, the content of linoleic acid in walnut oil increased from the original 8.7% to 95.9%. This suggests that the silver nitrate-silica gel column chromatography is a kind of high efficient separation method of linolenic acid.