直投式产γ-氨基丁酸植物乳杆菌冻干保护剂生产工艺的优化

Optimization of cryoprotectants production process for direct vat set Lactobacillus plantarum with high- yield of γ- amino butyric acid

摘要: 采用单因素实验和正交实验对产γ-氨基丁酸（γ-amino butyric acid,GABA）的植物乳杆菌Lp-Lw-131冻干保护剂进行筛选和优化,得到最佳保护剂配方为脱脂乳粉15.0g/L,蔗糖20.0g/L,麦芽糊精25.0g/L,甘油3.0m L/L。冻干后存活率为75.3%。将植物乳杆菌添加与未添加保护剂冻干后得到的菌粉进行电镜观察,添加保护剂的菌体结构和形态保持较好。

Abstract: A cryoprotectant formulation for Lactobacillus plantarum Lp- Lw-131 was optimized using the orthogonal experiment.The optimum cryoprotectant formulation was dried skim milk 15.0g / L,sucrose 20.0g / L,maltodextrin25.0g / L,glycerine 3.0m L / L. The survival rate of Lactobacillus plantarum with the addition of the optimized cryoprotectant formulation after freeze- drying was 75.3%.The electron microcopis observations showed that the protection agent chosen has good protection function on the bacteria.