Abstract: This study was aimed to evaluate the antioxidant activity of 25 kinds of pumpkin extraction in vitro. The scavenging of 1,1-diphenyl-2-picrylhydrazyl( DPPH) radicals and( 2,2'-even the ammonia( 3-ethyl benzene and thiazole-6-sulfonic acid) diamonium salt)( ABTS) radicals assay and ferric reducing capacity( FRAP) assay were used to measure the antioxidant activity of pumpkins. And BHT was used as the positive control drug. Results showed that the scavenging capability of DPPH free radical ability was weak.The ethyl acetate extract of Shanmei II showed the strongest power to clear ABTS radical( IC50=( 4.41 ± 0.23) μg/m L),better than that of BHT( IC50=( 8.59± 0.56) μg/m L).The power of ethyl acetate for reduction of Fe3 +ability was higher than that of other extracts,and Gailiangmiben ethyl acetate extract( TEAC =( 529.65 ± 69.52) μmol/g) was weaker than that of Trolox( TEAC =( 1387.51 ± 37.93) μmol/g).