Abstract: Three strains were isolated from the gut of healthy fish and identified by uniplex and multiplex PCR. The PCR results showed that length of 16 S rRNA was 350 bp, which were Blast in Gen Bank.Sequence analysis revealed that sample shared the highest sequence identity ( 90%) with similar genes which had been reported in Asia. The Aeromonas aerosol gene ( aer) and Aeromonas hydrophila hemolysin genes ( AHH) were also amplified, two length of 300 bp and 130 bp nucleotide sequences were obtained which shared more than 90% identity with Aeromonas hydrophila gene fragments which had been reported. However, the length of A. sobria hemolysin gene were not obtained.The results showed that the three isolations were all Aeromonas hydrophila which were Pathogenicity.