Abstract: In order to study the protective effects of torularhodin on oxidative injured PC12 cell. PC12 cells were randomly divided into seven groups: control group and model group ( 200μmol / L H2O2) , lycopene group ( positive control group) and treatment groupsⅠ- Ⅳ were added with torularhodin ( 1, 2, 3, 4μmol / L) under oxidative stress, respectively.The change of cell viability and cytotoxicity was observed by MTT assay and lactic dehydrogenase kit; the activities of antioxidant enzymes and malondialdehyde ( MDA) production were measured by enzyme linked immunosorbent assay; the accumulation of reactive oxygen species ( ROS) was detected by flow cytometric analysis.Results showed that in treatment groups Ⅰ- Ⅳ cell viability ( p < 0.01) was obviously increased and cell cytotoxicity ( p < 0.05) was decreased, and the activities of antioxidant enzymes ( p < 0.05) were significantly improved and the content of MDA ( p < 0.05) was reduced, and the accumulation of ROS was inhibited. What's more, treatment group Ⅲ had the best antioxidant effect. Torularhodin had protective effects on oxidative injured PC12 cell.While torularhodin was graded to four concentrations, 3μmol / L torularhodin achieved a better ability to resist oxidative stress.