Abstract: The ultrasonic- assisted extraction of polyphenols from Stauntonia chinensis was optimized using response surface methodology. Based on the single factor experimental analysis, Box- Behnken experimental design was used to explore the optimal processing conditions to extract polyphenols from Stauntonia chinensis including ultrasonic treatment time, ultrasonic temperature, acetone concentration and solid- to- solvent ratio. The results showed that the optimal extraction conditions were ultrasonic treatment time of 20 min, ultrasonic temperature of 63℃, acetone concentration of 58% and solid- to- solvent ratio of 1∶20 ( g / m L) .Through certification, the extraction yield of polyphenols from Stauntonia chinensis was 8.402 mg / g under the optimal conditions. In conclusion, the optimized extraction process is reliable and can be utilized to develop products based on Stauntonia chinensis polyphenols.