Abstract: In order to investigate the separation efficiency of macroporous resin on hawthorn flavonoids, ten types of macroporous resins was used to study the static adsorption properties on hawthorn flavonoids, after the optimum macroporous resins were deserved, D101 was seen as the optimal resin by the analysis of dynamic adsorption process of hawthorn flavonoids, the results indicate that static adsorption ratio of hawthorn flavonoids was 88.1%, desorption ratio was 92.9%. The optimized dynamic adsorption condition was hawthorn flavonoid concentration 18.75mg/mL, pH3 ~4, loading rate 1BV/h, volume fraction of ethanol 75%, adsorption ratio 1BV/h. The resin adsorption equilibrium was deserved when the sample volume was 6BV, and elution could be finished when the amount of eluent achieved 3BV. Under this condition, the purity of total concentration of rutin, hyperin and quercetin of hawthorn extract was 65.47%, and the recovery ratio was 86.22%.