Abstract: The sweetpatato starch was used as raw material, in order to determine the optimum technological conditions of sweetpotato resistant starch by microwave-enzyme method, the single factor experiment and response surface experiment were carried out. Results showed that best technological conditions were as follows:starch quality score 11%, microwave time 300 s, microwave effect power 800 W, pullulan enzyme adding amount of 78ASPU/g dry starch, debranching processing time 24 h, Under such conditions to repeat validation, RS yield was up to 31.25%, it might provide certain reference for resistant starch of sweet potato preparation and application in the food industry.