Abstract: Momordica charantia saponins microcapsules were prepared by piercing-solidifying method with chitosan and alginate as carriers shell material. The sustained- release performance of the prepared microcapsules in mimic gastric and intestinal environments was investigated. Meanwhile, the activity of alpha glycosidase enzymes and alpha amylase of the microcapsules in mimic gastric and intestinal environments were observed. Results indicated that the microcapsules display good sustained-release performance in mimic gastric and intestinal environments. The release rate of sapoins could reach to 31.19% in 1h and 52.14% in 2h in simulated gastric fluid. The accumulated release of saponins from microcapsules was 81.20% after 2h in simulated gastric fluid and 10 h in simulated intestinal fluid. Both alpha glycosidase and alpha amylase could be inhibited by microcapsules in mimic gastric and intestinal environments. With the increase of the release, there was a rising trend of inhibition ratio. Momordica charantia extracted microcapsules could protect saponins' activity, helped ease side effects and prolonged the acting time of glycosidase.