太平洋磷虾磷脂提取工艺研究及脂肪酸组成分析

Research of the optimization of extraction process of Euphausia pacifica phospholipid and analysis of fatty acids composition

摘要: 本研究以太平洋磷虾为原料,着重探讨了乙醇浓度、液固比、提取温度、提取时间对磷虾磷脂得率及纯度的影响。结合单因素实验与正交实验分析,结果表明:最佳工艺条件为乙醇浓度95%、液固比6mL/g,提取时间4h,提取温度40℃,在此工艺条件下粗磷脂得率为17.58%,纯度为46.09%。再经4℃丙酮沉降后,磷脂纯度为78.85%。产物通过气相色谱分析,不饱和脂肪酸占52.46%,在多不饱和脂肪酸中EPA和DHA含量之和最高,其占总脂肪酸含量的24.51%。

Abstract: The effects of ethanol concentration, the ratios of solvent to krill sample, extraction temperature and extraction time on the yield and purity of phospholipids extracted from Euphausia pacifica was investigated.Judging from the results of single factor experiment and orthogonal experiment, the optimal processing conditions were as follows: 95% ethanol, 6 to 1 ratio of solvent to krill sample, a 4h extraction time and a 40℃ extraction temperature.Under such conditions, the reasonable yield of 17.58% and purity of 46.09% were obtained.The purity reached 78.85% with a further purification by acetone at 4℃.The fatty acids of the further purified phospholipids were then analysed by GC.Results showed that the unsaturated fatty acids were 52.46%, the total percentage of EPA and DHA were 24.51% among all the fatty acids analysed.