四角蛤蜊醇沉上清液对CCl4致小鼠急性肝损伤保护作用
Experimental study on ethanol subsiding method from Mactra veneriformis on acute hepatic injury induced by carbon tetrachloride in mice
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摘要: 目的:研究四角蛤蜊醇沉上清液对四氯化碳致小鼠急性肝损伤的保护作用。方法:四氯化碳造成急性肝损伤模型,测定小鼠血清谷丙转氨酶(ALT)和谷草转氨酶(AST),还原性谷胱甘肽(GSH)活性;测定肝匀浆中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活力,对肝组织进行病理学检查。结果:醇沉上清液(MS)高、中、低剂量组具有明显的剂量依赖性降低CCl4致小鼠肝损伤血清ALT,AST,GSH(p<0.01),高、中剂量组降低肝匀浆中MDA的含量(p<0.05),增强SOD的活性(p<0.01)。通过病理学切片观察,MS各剂量组能显著改善肝组织的病理变化。结论:MS对CCl4造成的小鼠急性肝损伤具有显著的保护作用。Abstract: Objective:The effect of ethanol subsiding method from Mactra veneriformis (MS) was studied by carbon tetrachloride (CCl4) -induced liver injury in mice. Methods:Blood tissue samples were collection. Evaluations were made for lipid peroxidation, enzyme activities and biochemical parameters. Pathological histology was also performed. Results :MS pretreatment reduced the serum aspartate aminotransferase (ALT) , alanine aminotransferase (AST) , and reduced glutathione activities. By contrast, MS pretreatment reduced liver MDA content and increased liver tissue SOD. The CCl4-induced histopathological changes were also reduced by the MS pretreatment. Conclusion:MS could be proposed to protect the liver damage induced by CCl4.
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