酶-超声波联用提取紫薯色素的工艺研究
Study on enzyme-ultrasonic combined extraction method of purple sweet potato pigment
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摘要: 研究了酶-超声波联用提取紫薯色素的工艺条件,通过单因素和L9(34)正交实验确定了其最佳提取工艺条件为:纤维素酶用量20IU/g,酶解时间45min,酶解温度50℃,酶解pH5.6,料液比1∶10(g/mL),超声时间20min,超声温度60℃。在最佳提取工艺条件下,最佳提取次数为三次时,紫薯色素的提取率可达98.7%。同时对比了酶法、超声法、酶-超声联用法三种提取方法,结果表明酶-超声波联用法的紫薯色素提取率较酶法、超声法分别提高了39.3%和20.3%。研究表明酶-超声波联用法能更好地提取紫薯色素,为紫薯色素的提取提供了一种新工艺。Abstract: The enzyme-ultrasonic combined extraction method of purple sweet potato pigment was studied by single factor experiment and L9 (34) orthogonal experiment. The results showed that the optimum extraction conditions were obtained as follows:cellulase dose 20IU/g, enzymatic hydrolysis time 45min, enzymatic hydrolysis temperature 50℃, enzymatic hydrolysis pH5.6, solid-liquid ratio 1 ∶10, ultrasonic time 20min and ultrasonic temperature 60℃. Under the optimum conditions, the extraction rate reached up to 98.7% after three times extractions. Furthermore, the three extraction methods (enzymatic extraction, ultrasonic extraction and enzymeultrasonic combined extraction) were compared. The pigment extraction rate of enzyme-ultrasonic combined extraction method increased by 39.3% and 20.3% respectively compared to that of enzyme extraction and ultrasonic extraction. The results of this study indicated that pigment extraction rate of enzyme-ultrasonic combined extraction method was the highest. This study would provide a new extraction method for purple sweet potato pigment.