Abstract: Drink formula of chrysanthemum and medlar was optimized and its antioxidant activity was determined.By D-optimal mixture design, the best drink formula of chrysanthemum and medlar was:30% of chrysanthemum extract, 51% of medlar extract and 19% of soft sugar solution. In this condition, the maximum value of sensory evaluation of drink was 86.20. At concentration of 100%, the drink could eliminated 67.97% DPPH radicals and51.15% hydroxyl radicals and showed strong antioxidant activity. This drink was yellow, sweet and sour moderate, taste lubrication, with rich chrysanthemum and medlar fragrance. Thus, this drink would be a green, nutritive and healthy functional beverage.