Abstract: ObjectiveTo study the solid culture conditions of antrodia subxantha mycelium and test the CAT and SOD activity of the mycelium and fermentation liquor with it 's growth period.MethodsThe effect of carbon sources, nitrogen sources, mineral salts, temperature and light on the mycelial growth of Antrodia subxantha was researched, according to the mycelial growth rate and the growth vigor.Ultraviolet absorption method and NBT light reduction method were used to test the activity of CAT and SOD.ResultsThe optimum carbon source was glucose, the optimum nitrogen source was yeast powder. The mineral salts used in the trial do not promote the mycelial growth obviously.The optimal temperature was from 25 to 35℃.It growth better in darkness entirely as well as darkness with light alternately.The activity of CAT and SOD in mycelium had little regularity.CAT was not found in the fermentation liquor.While the activity of SOD in the fermentation liquor increased after decreasing for a period.ConclusionsSolid culture condition was founded initially.The activity of CAT and SOD with the processing of culture was tested.