Abstract: To optimize the microwave-assisted extraction for polyphenols in Mango core. Antibacterial activity of polyphenols was also studied. Yield of polyphenols rate as indicators, ethanol concentration, ratio of material to liquid, microwave power and extraction time on extraction rate of mango core polyphenols were investigated by single factor and orthogonal test. The filter paper disc were used in bacterial sensitivity test in vitro. The optimize extraction process conditions as follows:ratio of material to liquid 1∶20 (g/mL) , extraction time 60 s, microwave power 340 W, ethanol concentration 60%, and that Mango core ployphenols yield was up to 9.27%±0.54%. Mango core ployphenols had bacteriostasis activity in vitro, especially against the staphylococcus epidermidis. The study could provide scientific basis for antioxidant and antibacterial activity of phenolics from mango, which could be used to develop the economic value of mango core.