Abstract: Response surface method and the Design-Expert 8 were used to optimize the fermentation conditions. In the research, Inulin Granules, Inulin and Glucose were as the carbon source culture medium, which influenced the growth of the Lactobacillus bulgaricus (ASI.1482) , indicating that the excellent fermentation capability of the Inulin Granules. The results indicated that Inulin Granules or Inulin as the single carbon source media would have the proliferation effect on the lactobacillus, and this effect would be prior to that of the Inulin and Glucose with the lactobacillus proliferation of 4.8 times of Inulin and 9.6 times of Glucose. Inulin Granules was with the possibility of Lactobacillus bulgaricus carbon source culture medium. Its optimal fermentation conditions for carbon was 2.1% of source added volume, 36℃ of fermentation temperature, and 10% vaccination rates. In such conditions, the count of Lactobacillus bulgaricus colonies was total for 2.2×109CFU/mL.