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中国精品科技期刊2020
晋文慧, 陈伟珠, 洪专, 张怡评, 方华, 谢全灵. 超高效液相色谱法测定藻油中的DPA和DHA[J]. 食品工业科技, 2014, (09): 278-281. DOI: 10.13386/j.issn1002-0306.2014.09.052
引用本文: 晋文慧, 陈伟珠, 洪专, 张怡评, 方华, 谢全灵. 超高效液相色谱法测定藻油中的DPA和DHA[J]. 食品工业科技, 2014, (09): 278-281. DOI: 10.13386/j.issn1002-0306.2014.09.052
JIN Wen-hui, CHEN Wei-zhu, HONG Zhuan, ZHANG Yi-ping, FANG Hua, XIE Quan-ling. Rapid detection of docosapentenoic acid and docosahexaenoic acid in algae oil by ultra performance liquid chromatography[J]. Science and Technology of Food Industry, 2014, (09): 278-281. DOI: 10.13386/j.issn1002-0306.2014.09.052
Citation: JIN Wen-hui, CHEN Wei-zhu, HONG Zhuan, ZHANG Yi-ping, FANG Hua, XIE Quan-ling. Rapid detection of docosapentenoic acid and docosahexaenoic acid in algae oil by ultra performance liquid chromatography[J]. Science and Technology of Food Industry, 2014, (09): 278-281. DOI: 10.13386/j.issn1002-0306.2014.09.052

超高效液相色谱法测定藻油中的DPA和DHA

Rapid detection of docosapentenoic acid and docosahexaenoic acid in algae oil by ultra performance liquid chromatography

  • 摘要: 采用超高效液相色谱(UPLC)方法快速分析测定藻油中DPA甲酯和DHA甲酯的含量,考察了流动相、流速及柱温等条件对藻油中DPA-ME和DHA-ME分离的影响,确定了最佳色谱条件:等度洗脱,流动相为乙腈-水(80∶20,V/V),流速0.5mL/min,检测波长205nm。该方法下,二十二碳五烯酸甲酯(DPA-ME)和二十二碳六烯酸甲酯(DHA-ME)分别在10.2510.0、11.1554.0μg/mL范围内浓度和峰面积呈现良好的线性关系,相关系数r均为0.9999;平均回收率分别为99.1%(RSD=1.3%)和100.6%(RSD=1.4%)。本方法快速、灵敏、重复性好,适合于藻油中DPA和DHA含量的检测。 

     

    Abstract: In order to determine the content of docosapentenoic acid ( DPA) and docosahexaenoic acid ( DHA) in algae oil quickly and efficiently, a rapid ultra- high pressure liquid chromatography ( UPLC) method was developed.The influence of mobile phase, flow rate on the separation of DPA- ME and DHA- ME in algae oil were comprehensively studied.The optimal separation conditions were as follows: a mobile phase consisting of acetonitrile and water ( 80∶20, V/V) with a isocratic elution profile, UV detection wavelength at 205nm, 0.5mL /min of flow rate for mobile phase.On the above conditions, good linearity between response ( peak area) and concentration was found over a concentration range of 10.2510.0μg /mL for DPA- ME, and 11.1 554.0μg /mL for DHA- ME respectively.And the correlation coefficient of the calibration curve of DPA- ME and DHA- ME was 0.9999.The average recovery rates of DPA-ME and DHA-ME were 99.1% ( RSD = 1.3%) and 100.6% ( RSD = 1.4%) respectively.This method was high- speed, sensitive and with a good reproducibility.It was appropriate for fast detection of DPA and DHA in algae oil.

     

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