Abstract: Objective: the use of alcohol to dissolve cholesterol was discussed and a way to lower cholesterol in the duck intestine was provided.Methods: different alcohol concentration, material- liquid ratio, soaking time were chosen and then combine single factor experiment with response surface analysis to optimize the best condition to removal of cholesterol in duck intestine, cholesterol content used spectrophotometry to measure.Results: through the experiment the best process conditions to removal of cholesterol in duck intestine was material- liquid ratio of 1∶8, alcohol concentration was 23.56%, soaking time was 2.7h.Under the condition, the duck intestinal cholesterol removal rate was 44.64%.Conclusion: edible alcohol method can effectively remove the cholesterol in duck intestine.