Abstract: Methionol is an important volatile sulphur flavor compound.It can be produced in Saccharomyces cerevisiae using L- methionine by the Ehrlich pathway.To verify aminotransferase'effect, gene ARO8 encoding aminotransferase was cloned from S.cerevisiae S288C, and the gene ARO8 was inserted into the S.cerevisiaeE.coli shuttle constitutive expression vector pYES- pgk, generating a recombinant vector pYES- pgk- ARO8.The vector pYES- pgk- ARO8 was then transformed into S.cerevisiae S288C.The results indicated that the encoding gene shared 100% homology with the aromatic aminotransferase Ⅰ gene ARO8 reported in NCBI GenBank.A recombinant strain S.cerevisiae AR8 was constructed and screened successfully.The aminotransferase activity of S.cerevisiae AR8 was 187U /mL, which was increased by 28.8% compared to what in S.cerevisiae S288C.Methionol yield of AR8 in shaking flask reached 0.76g /L and it was increased by 28.8%.These results showed that aminotransferase Aro8p and gene ARO8 of S.cerevisiae played an important role in methionol anabolism, and the gene ARO8 over-expression was help to methionol yield.