Abstract: The antioxidant activity of three kinds of Pu-erh teas prepared by solid fermentation with using sundried green tea, semi- fermentation black tea and black tea as materials was investigated in this study.The results showed that the water extracts of three kinds of Pu- erh teas had strong antioxidant activity and showed a good dose-effect relationship.The DPPH· scavenging effect, reducing power, ·OH scavenging effect and inhibition of lipid peroxidation of Pu- erh tea prepared from semi- fermentation black tea were significantly higher than other Pu-erh tea. It suggested that semi-fermentation black tea can be used as material for preparation of Pu-erh tea with high antioxidant activity.