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中国精品科技期刊2020
苏卿, 李谦, 陈浩, 张文众, 李宁, 贾旭东, 周志钦. 叶黄素的抗氧化促氧化作用研究[J]. 食品工业科技, 2014, (09): 68-71. DOI: 10.13386/j.issn1002-0306.2014.09.005
引用本文: 苏卿, 李谦, 陈浩, 张文众, 李宁, 贾旭东, 周志钦. 叶黄素的抗氧化促氧化作用研究[J]. 食品工业科技, 2014, (09): 68-71. DOI: 10.13386/j.issn1002-0306.2014.09.005
SU Qing, LI Qian, CHEN Hao, ZHANG Wen-zhong, LI Ning, JIA Xu-dong, ZHOU Zhi-qin. Anti-oxidative and pro-oxidative character study of lutein[J]. Science and Technology of Food Industry, 2014, (09): 68-71. DOI: 10.13386/j.issn1002-0306.2014.09.005
Citation: SU Qing, LI Qian, CHEN Hao, ZHANG Wen-zhong, LI Ning, JIA Xu-dong, ZHOU Zhi-qin. Anti-oxidative and pro-oxidative character study of lutein[J]. Science and Technology of Food Industry, 2014, (09): 68-71. DOI: 10.13386/j.issn1002-0306.2014.09.005

叶黄素的抗氧化促氧化作用研究

Anti-oxidative and pro-oxidative character study of lutein

  • 摘要: 以叶黄素为受试物,采用抗氧化能力评价化学方法,包括氧自由基吸收能力法(ORAC方法)、Trolox Equivalent Antioxidant Capacity Assay(TEAC法)、Folin-Ciocalteu法(FCR实验法)、1,1-二苯基-2-三硝基苯肼法(DPPH法),同时采用评价生物大分子损伤的脱细胞彗星实验,对叶黄素的抗氧化-促氧化作用进行探索。结果表明,ORAC法和DPPH法表明不同浓度叶黄素分别呈现出抗氧化和促氧化的双重作用;TEAC法表明叶黄素具有抗氧化作用、FCR实验表明叶黄素含有酚类物质较少,酚类物质不是叶黄素抗氧化作用的主要因素;脱细胞彗星实验表明高剂量叶黄素对DNA分子具有损伤。因此,叶黄素在不同浓度表现出抗氧化—促氧化的双重作用。 

     

    Abstract: In the study, lutein was used as the test material, chemical methods including Oxygen Radical Absorbance Capacity ( ORAC) , Trolox Equivalent Anti- oxidant Capacity assay ( TEAC) , Folin- Ciocalteu Reaction ( FCR) , 1, 1-Diphenyl-2-picrylhydrazylradical2, 2-Diphenyl-1- ( 2, 4, 6-trinitrophenyl) hydrazyl ( DPPH) were used to evaluate the anti- oxidative character of lutein, and at the same time, the method of evaluating biological macromolecular injury- acellular comet assay was applied in this study.Different concentrations of lutein was demonstrated to have anti-oxidation and pro- oxidation in the ORAC and DPPH; lutein was proved to have antioxidation in TEAC; lutein was indicated to contain less phenolics, and the phenolics was not the main factor of anti-oxidant effect in FCR; high concentration of lutein had damage to DNA in the acellular comet assay.Therefore, lutein showed double effect of anti-oxidant and pro-oxidant in different concentration.

     

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