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中国精品科技期刊2020
董梅, 李亚辉, 樊明涛, 李爱霞, 王盼雪. 酒类酒球菌31MBR β-D-葡萄糖苷酶超声提取工艺优化[J]. 食品工业科技, 2014, (05): 163-166. DOI: 10.13386/j.issn1002-0306.2014.05.088
引用本文: 董梅, 李亚辉, 樊明涛, 李爱霞, 王盼雪. 酒类酒球菌31MBR β-D-葡萄糖苷酶超声提取工艺优化[J]. 食品工业科技, 2014, (05): 163-166. DOI: 10.13386/j.issn1002-0306.2014.05.088
DONG Mei, LI Ya-hui, FAN Ming-tao, LI Ai-xia, WANG Pan-xue. Optimization of ultrasonic conditions for β-Glucosidase extraction from Oenococcus oeni 31MBR[J]. Science and Technology of Food Industry, 2014, (05): 163-166. DOI: 10.13386/j.issn1002-0306.2014.05.088
Citation: DONG Mei, LI Ya-hui, FAN Ming-tao, LI Ai-xia, WANG Pan-xue. Optimization of ultrasonic conditions for β-Glucosidase extraction from Oenococcus oeni 31MBR[J]. Science and Technology of Food Industry, 2014, (05): 163-166. DOI: 10.13386/j.issn1002-0306.2014.05.088

酒类酒球菌31MBR β-D-葡萄糖苷酶超声提取工艺优化

Optimization of ultrasonic conditions for β-Glucosidase extraction from Oenococcus oeni 31MBR

  • 摘要: 为了更有效地提取β-D-葡萄糖苷酶,在单因素实验基础上,通过响应面分析优化了酒类酒球菌31MBRβ-D-葡萄糖苷酶的提取工艺,确定了利用超声波破碎细胞提取β-D-葡萄糖苷酶的最佳条件如下:破碎时间20min,破碎功率130W,菌体浓度OD600=2.0。在此条件下得到的31MBR粗酶液的总酶活为13.89U,明显高于反复冻融法和玻璃珠破碎法所得粗酶液的酶活。超声波破碎可以有效地提取酒类酒球菌葡萄糖苷酶,运用响应面分析方法优化超声波破碎条件是可行的。 

     

    Abstract: In order to extractβ- D- Glucosidase from Oenococcus oeni 31MBR effectively, response surface methodology was used to optimize ultrasonic conditions on the basis of single factor test.The results showed that optimum conditions were: ultrasonic time 20min, ultrasonic power 130W, and the cell concentration about OD600=2.0. Under such conditions, totalβ- D- glucosidase activity from 31MBR was about 13.89U, which was higher than repeated freezing and melting and glass- bead. β- D- Glucosidase could be extracted from Oenococcus oeni effectively by ultrasonic, and it was feasible to optimize ultrasonic extraction conditions using response surface methodology.

     

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